Journal: Advanced Healthcare Materials

Article Title: Plasma‐Polymerized Nanoparticles Presenting Fibrillin‐1 Drive Rapid Re‐Endothelialization of Vascular Grafts

doi: 10.1002/adhm.202503360

Figure Lengend Snippet: PPN‐PF8 functionalization of ePTFE improves endothelial attachment in vitro. A) Representative images of SEM and F‐actin staining. B) HCAEC attachment and C) spreading on ePTFE, ePTFE with passively bound PF8 (ePTFE‐PF8), PPN‐coated ePTFE (PPN), and PPN‐PF8 functionalized ePTFE, n = 3 per sample. Scale bar = 300 µm. * p < 0.05, *** p < 0.001 versus ePTFE.

Article Snippet: Human coronary artery endothelial cells (HCAEC; Cell Applications) were cultured in MesoEndo medium (Cell Applications) at 37 °C in 5% CO 2 .

Techniques: In Vitro, Staining

Journal: Advanced Healthcare Materials

Article Title: Plasma‐Polymerized Nanoparticles Presenting Fibrillin‐1 Drive Rapid Re‐Endothelialization of Vascular Grafts

doi: 10.1002/adhm.202503360

Figure Lengend Snippet: PPN‐PF8 functionalization of ePTFE improves endothelial proliferation in vitro. A) Representative images of SEM and F‐actin staining of HCAEC proliferation on days 1 and 3. B) HCAEC proliferation and C) spreading on ePTFE, ePTFE with passively bound PF8, PPN, and PPN‐PF8 functionalized ePTFE. * p < 0.05, *** p < 0.001 versus ePTFE on each day, Scale bar = 300 µm. n = 3 per sample.

Article Snippet: Human coronary artery endothelial cells (HCAEC; Cell Applications) were cultured in MesoEndo medium (Cell Applications) at 37 °C in 5% CO 2 .

Techniques: In Vitro, Staining

Journal: Circulation. Cardiovascular interventions

Article Title: Impact of stent surface on thrombogenicity and vascular healing: a comparative analysis of metallic and polymeric surfaces.

doi: 10.1161/CIRCINTERVENTIONS.113.000120

Figure Lengend Snippet: Figure 4. Endothelialization of uncoated and polyvinylidene flu- oride-co-hexafluoropropene (PVDF-HFP)–coated platinum chro- mium (PtCr) stents. A, Absorbance values of CD31 expression (minus background) for endothelial cells (ECs) covering stents at 7 (n=9) and 14 days (n=7). At 7 (P<0.01) and 14 (P=0.02) days, there were significant differences between the endothelialization between uncoated and PVDF-HFP–coated PtCr stents. Values shown are mean±SEM . B, Human coronary artery EC coverage on PVDF-HFP at 7 (n=9) and 14 days (n=7) was normalized to the respective value for PtCr at each time point. Endothelialization of uncoated PtCr stents was greater at 7 and 14 days compared with that on PVDF-HFP–coated stents (P<0.01). Values shown are mean with 95% confidence interval. *Significantly different from PtCr at the respective time point (P<0.05).

Article Snippet: Endothelial cell (EC) growth on uncoated and PVDF-HFP–coated PtCr stents (3.0 mm×16 mm, ELEMENT) was evaluated using human coronary artery ECs (HCAECs; Cell Applications Inc, San Diego, CA), as previously described,11 and is described in detail in the online-only Data Supplement.

Techniques: Expressing

Journal: Circulation. Cardiovascular interventions

Article Title: Impact of stent surface on thrombogenicity and vascular healing: a comparative analysis of metallic and polymeric surfaces.

doi: 10.1161/CIRCINTERVENTIONS.113.000120

Figure Lengend Snippet: Figure 5. Representative micrographs of immunohistochemical staining of human coronary artery endothelial cells (HCAECs) on uncoated and polyvinylidene fluoride-co-hexafluoropropene (PVDF-HFP)–coated platinum chromium (PtCr) stents at 14 days. 4',6-diamidino-2-phenylindole (DAPI)–stained nuclei of HCAEC on (A) uncoated and (D) PVDF-HFP–coated stents demonstrate presence of cells at 14 days with greater levels on uncoated stents. Expression of vascular endothelial (VE)-cadherin at intracellular junctions of HCAECs on (B) uncoated stents is readily evident and reflects typical cobblestone morphology of endothelial cells (within circle). In contrast, VE-cadherin expression on HCAEC covering (D) PVDF-HFP–coated stents is relatively weaker at intracellular junctions (within circle), sug- gesting reduced barrier function compared with HCAEC covering uncoated stents. For comparison on uncoated PtCr stents, (H) an isotype control for VE-cadherin displays nominal back- ground staining. C, F, and I, DAPI and VE-cadherin images were merged with the transmitted images of the underlying stents to represent strut location.

Article Snippet: Endothelial cell (EC) growth on uncoated and PVDF-HFP–coated PtCr stents (3.0 mm×16 mm, ELEMENT) was evaluated using human coronary artery ECs (HCAECs; Cell Applications Inc, San Diego, CA), as previously described,11 and is described in detail in the online-only Data Supplement.

Techniques: Immunohistochemical staining, Staining, Expressing, Comparison, Control

Journal: Molecular Medicine Reports

Article Title: X-irradiation induces acute and early term inflammatory responses in atherosclerosis-prone ApoE − / − mice and in endothelial cells

doi: 10.3892/mmr.2021.12038

Figure Lengend Snippet: Inflammatory response in endothelial cells at 24 h after irradiation. The response of various inflammatory markers at 24 h after 0.1 and 5 Gy irradiation is represented in (A-I) TICAE cells and (J-R) TIME cells, normalized to cell count. The Kruskal-Wallis test was used to analyse the data and the P-value was adjusted using the Benjamini-Hochberg method. Values represent the average ± SEM of 6 biological replicates. *P<0.05, **P<0.01 and ***P<0.001 vs. 0 Gy. p.i., post irradiation; GDF-15, growth differentiation factor-15; CXCL10, C-X-C motif chemokine ligand 10; ICAM-1, intercellular adhesion molecule-1; MCP-1, monocyte chemoattractant protein-1; uPAR, urokinase-type plasminogen activator receptor; PAI-1, plasminogen activator inhibitor-1; FGF-basic, basic fibroblast growth factor.

Article Snippet: In addition, TICAE cells, which are primary human coronary artery endothelial cells from the European Collection of Authenticated Cell Cultures (ECACC; HCAECs cat. no. 300-05a) that were transduced with retroviruses bearing the est2 gene, a yeast homologue of the human TERT protein ( , ), were used.

Techniques: Irradiation, Cell Counting

Journal: Molecular Medicine Reports

Article Title: X-irradiation induces acute and early term inflammatory responses in atherosclerosis-prone ApoE − / − mice and in endothelial cells

doi: 10.3892/mmr.2021.12038

Figure Lengend Snippet: Inflammatory response in endothelial cells at 72 h after irradiation. The response of various inflammatory markers at 72 h after 0.1 and 5 Gy irradiation is presented in (A-I) TICAE cells and (J-R) TIME cells. Data were normalized to cell count. The Kruskal-Wallis test was used to analyse the data and the P-value was adjusted using the Benjamini-Hochberg method. Values represent the average ± SEM of 6 biological replicates. *P<0.05, **P<0.01 and ***P<0.001 vs. 0 Gy. p.i., post irradiation; GDF-15, growth differentiation factor-15; CXCL10, C-X-C motif chemokine ligand 10; ICAM-1, intercellular adhesion molecule-1; MCP-1, monocyte chemoattractant protein-1; uPAR, urokinase-type plasminogen activator receptor; PAI-1, plasminogen activator inhibitor-1; FGF-basic, basic fibroblast growth factor.

Article Snippet: In addition, TICAE cells, which are primary human coronary artery endothelial cells from the European Collection of Authenticated Cell Cultures (ECACC; HCAECs cat. no. 300-05a) that were transduced with retroviruses bearing the est2 gene, a yeast homologue of the human TERT protein ( , ), were used.

Techniques: Irradiation, Cell Counting